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KMID : 0545120160260030503
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Journal of Microbiology and Biotechnology
2016 Volume.26 No. 3 p.503 ~ p.510
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Magnesium Uptake by the Green Microalga Chlorella vulgaris in Batch Cultures
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Ayed Hela Ben Amor-Ben
Taidi Behnam
Ayadi Habib
Pareau Dominique
Stambouli Moncef
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Abstract
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The accumulation (internal and superficial distribution) of magnesium ions (Mg2+) by the green freshwater microalga Chlorella vulgaris (C. vulgaris) was investigated under autotrophic culture in a stirred photobioreactor. The concentrations of the three forms of Mg2+ (dissolved, extracellular, and intracellular) were determined with atomic absorption spectroscopy during the course of C. vulgaris growth. The proportions of adsorbed (extracellular) and absorbed (intracellular) Mg2+ were quantified. The concentration of the most important pigment in algal cells, chlorophyll a, increased over time in proportion to the increase in the biomass concentration, indicating a constant chlorophyll/biomass ratio during the linear growth phase. The mean-average rate of Mg2+ uptake by C. vulgaris grown in a culture medium starting with 16 mg/l of Mg2+ concentration was measured. A clear relationship between the biomass concentration and the proportion of the Mg2+ removal from the medium was observed. Of the total Mg2+ present in the culture medium, 18% was adsorbed on the cell wall and 51% was absorbed by the biomass by the end of the experiment (765 h). Overall, 69% of the initial Mg2+ were found to be removed from the medium. This study supported the kinetic model based on a reversible first-order reaction for Mg2+ bioaccumulation in C. vulgaris, which was consistent with the experimental data.
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KEYWORD
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Chlorella vulgaris, absorbed Mg2+ (intracellular), metal ions uptake, photo-bioreactor, adsorbed Mg2+ (extracellular)
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